What
did we Conclude?
Overall,
our experiments did not work out as well as we had hoped, and less than half of
the information that we should have gotten actually came out. This could be due
to improper procedure when using the Laser Capture Microdissector, not having
enough DNA for the Electrophoresis machine to process, or using the wrong
concentrations of materials for this sort of sample. Another possibility could
have been an error in pipetting, but this is unlikely because the experiment
was repeated and re-pipetted and the results were similarly lacking. However,
we did get enough data to conclude that microheterogeneity does exist, and that
it is worthwhile to check for microheterogeneity when analyzing a tumor because
if there is microheterogeneity, treatment may be different.
The most rewarding aspect of this
project was the opportunity to experience actual lab work. It was nice to be
able to see what it would really be like to work in a research laboratory. This was especially helpful because choosing
a field to go into is often difficult when you don't know too much about it. This
is especially true since we have both been considering research as a possible
option. Overall, the opportunity to experience it was invaluable, especially
because it was something we really enjoyed.
This project turned out to be one of
the most successful group projects either of us had ever done. We worked well
together, and our productive mesh of talents produced a product better then that
which would have been seen had we worked alone.